Tylosensor is a competitive test involving a specific antibody in one single operation.
The test requires the use of two components :
The first component is a microwell containing predetermined amounts of antibodies linked to gold particles.
The second is a dipstick made up of a set of membranes with specific capture lines.
For a valid test, the upper red control (“CTRL”) line has to be visible after the second incubation. The other line is the Tylosin-A specific test (“TEST”) and is located below the control line. When the reagent from the microwell is re-suspended with a milk sample, all antibodies will bind the corresponding analytes if present during the first 3- minute incubation at 40°C. Afterwards, when the dipstick is dipped into the milk, the liquid starts running vertically on the dipstick and passes through capture zones. When the sample is free of antibiotics, a colour development occurs at the specific capture lines, indicating the absence of the targeted analytes in the milk sample. On the contrary, the presence of antibiotics in the sample will not cause any coloured signal to appear at the specific capture lines.
Tylosin is a macrolide antibiotic used in veterinary medicine to treat bacterial infections in a wide range of species. It has a broad spectrum of activity against Gram-positive organisms. However, antibiotic residues in milk above tolerance levels interfere with dairy product processing and pose potential health risks to consumers.